VSV virus vector
Vesicular stomatitis virus (VSV) vectors are widely used in studying the invasion of viral particles into host cells, identifying cell surface receptors that mediate viral infection of cells, screening for viral inhibitory drugs, and vaccine development.VSV viral vectors can efficiently use the pseudotyping of the envelope proteins of heterologous viruses, such as those that need to be operated in a high biosafety grade laboratory.Recombinant VSV cannot be used for more than one round of replication in host cells and can therefore be manipulated in a biosafety level 2 (BSL 2) laboratory. These features make VSV viral vectors ideal for studying the cellular mechanisms of high-risk virus invasion without high isolation.Wild-type VSV belongs to the Rhabdoviridae, with a single-stranded negative-sense RNA genome and its replication-virus-dependent RNA-dependent RNA polymerase (RdRp).The single-stranded RNA genome of VSV encodes the following five protein components: nuclear protein (N), phosphorylated protein (P), matrix protein (M), glycoprotein (G), and the large subunit (L) of the viral RdRp. In the VSV virus particles, its RNA genome and N nuclear protein constitute a ribonucleoprotein (RNP). The P and L proteins then compose the RdRp and bind to the RNP.When VSV infects target cells, its G glycoprotein induces the cells to undergo endocytosis, helping the virus to invade the cells. The G glycoprotein can mediate the fusion of the viral envelope with the cell membrane, prompting the release of the RNP and RdRp into the cytoplasm together. After the viral component enters the cell, RdRp initiates primary transcription using the negative-sense RNA genome in the RNP as the template, and the sense RNA strand as a transcript product is further translated to produce late proteins that mediate viral functions such as viral genome replication, secondary transcription, viral assembly and budding.
For more information on this carrier system, please refer to the following literature.
For more information on this carrier system, please refer to the following literature.
| Reference Documentation | Theme |
| Hum Vaccin Immunothera. 15:2269 (2019) | Recombinant vesicular stomatitis vector vaccines |
| Vaccine. 34:6597 (2016) | Risk/benefit assessment of live viral vaccines based on VSV |
| Front Microbiol. 2:272 (2012) | Development and applications of VSV vectors |
| J Virol Methods. 169:365 (2010) | Generation of VSV pseudotypes using recombinant VSV-delta G |
| J Gen Virol. 86:2269 (2005) | Generation of VSV pseudotyped with coronavirus spike protein |
| Virology. 286:263 (2001) | Characterization of VSV pseudotyped with HCV envelop proteins |
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